Histology

IMPORTANT: Histology services at Covid-19 Re-Entry operation

For detailed information on the current facility operations, please click here.

Histology – the power to reveal unexpected connections

Histology, the science of studying microscopic structure of the cells, tissues, and organs, provides crucial information to understand the relationship between structure and function of biological machinery.

The Histology Facility offers versatile equipment and expert knowledge that allow researchers to preserve and visualize microanatomy of their model organisms.

Our services encompass tissue processing, routine histological stains, a growing array of immunostaining techniques and in situ hybridization methods. We are dedicated to develop highly individualized approaches for each project.

Do you work with tissue systems or animal models? Do you study disease processes? Are you interested in our services?  

SERVICES

Experimental design and protocol planning

We can assist with study design, and development and implementation of protocols for standard as well as customized experimental studies.

Preanalytical procedures

We routinely provide services and support for:

  • Sample collection, fixation, and processing
  • Cryo- and paraffin embedding and sectioning
  • Staining

We have experience working with several types of samples including whole organisms, isolated organs, slices or groups of tissues, cell cultures (2D and 3D), fluids, and inorganic materials.

We perform routine and special histochemical stains along with immunostainings (immunofluorescence, immunohistochemistry, and multiplex stains). We have experience with background reduction in problematic “mouse on mouse” stainings as well as cross-reactivity avoidance during multiplex stainings. We also offer RNA in situ hybridization with basic protocols and RNAscope.

Auxiliary services

Support during self-service is offered exclusively to IMP/IMBA/GMI members. It includes training in the use of all the sectioning equipment available in the facility and troubleshooting support.

Secondary and inter-facility support includes coordinated processing services (e.g., perfusion and dissection after in vivo imaging) offered with other facilities for multimodal projects. For example, HistoPathology staff work collaboratively with the IMP Biooptics core facility to facilitate whole slide digitization (slide scanning).

EQUIPMENT

LabSat® Automated Stainer by Lunaphore

LabSat® is an automated stainer utilizing a microfluidic technology called FFeX and a pressurized system. It is capable of carrying out IHC/IF staining cycles within a few minutes, in a highly precise and reproducible manner. This highly open system allows for testing many types of reagents and conditions. Thus, the Histology Facility will be able to accelerate the optimization times of antibodies as well as introduce automated multiplexing immunostaining services on VBC campus.

BioGenex i6000 stainer

A fully open immunostaining system with the capacity for 60 slides per run. It can perform immunochemistry staining with either DAB or AP-red detection systems using customized protocols.

If you are interested in having immunostains done on your samples with the i6000, please contact us.

Automatic Tissue Processor Donatello

This instrument performs automatic tissue dehydration, clearing, and paraffin infiltration of up to 360 tissue cassettes in one run.

If you are interested in using the Donatello, please contact us.

CryoStar™ NX70 Cryostat

Microtome to section frozen or cryo-embedded tissues. Open-top cryostat with ergonomic design for working while standing or sitting. This cryostat allows a section thickness range from 0.5 to 100 µm. The temperature for the specimen clumper is controlled and ranges from 10°C to -35°C. There is a separate temperature control for the knife carrier down to -35°C.

Rotary microtome

Heavy duty microtomes used for paraffin sectioning, allowing a section thickness range from 0.5 to 100 µm. The sections can then be transferred onto a glass slide and used for a wide variety of staining protocols (basic staining, immunohistochemistry, in situ hybridization, or immunofluorescence).

Please click here for more equipment.

USER INFORMATION

To order a service, please contact the facility staff as early as possible to discuss relevant details and agree upon timelines. When submitting samples, please remember to label each sample with a unique identifier (e.g., mouse ID or organoid batch number), submitter initials, and date. Please also provide relevant details such as: genotype, treatment, or other procedure.I f procedures relevant to coordinated processing have been performed (e.g. in vivo imaging), please ensure that pertinent details and representative images are provided. The clearer and more complete the information provided, the lower the risk of downstream errors. The facility can also provide you with submission forms for your projects.

IMP, IMBA, and GMI researchers can, by themselves, use all sectioning equipment in the facility, but only after attending a training session with our staff. To set up an appointment for training, please contact the facility staff.  

In addition, the facility offers many for-fee-services to members of the Vienna BioCenter (academic institutions and companies) as well as external academic institutions and private companies.

For paid service projects, the users from the Vienna BioCenter have priority. Otherwise, the service projects are performed in the order they are requested; ‘first come, first served’.

We require acknowledgement of facility use in publications. 
A simple statement is sufficient and can be placed in the Materials and Methods section or in the Acknowledgments section, depending on the journal format.

Suggested format:

The XXXXXX was performed by the Histology Facility 
at Vienna BioCenter Core Facilities (VBCF), member of the Vienna BioCenter (VBC), Austria.

In case of (co-)authorship:

The Vienna BioCenter Core Facilities (VBCF) Histology Facility acknowledges funding from the Austrian Federal Ministry of Education, Science & Research; and the City of Vienna.

SCIENTIFIC CONTRIBUTIONS

Global biogeography of chemosynthetic symbionts reveals both localized and globally distributed symbiont groups. Osvatic JT, Wilkins LGE, Leibrecht L, Leray M, Zauner S, Polzin J, Camacho Y, Gros O, van Gils JA, Eisen JA, Petersen JM, Yuen B. Proc Natl Acad Sci U S A. 2021 118(29):e2104378118

Central amygdala circuitry modulates nociceptive processing through differential hierarchical interaction with affective network dynamics. Wank, I., Pliota, P., Badurek, S., Kraitsy, K., Kaczanowska J., Griessner J., Kreitz S., Hess A., Haubensak W. Commun Biol 2021 4, 732.

Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly. Krenn V, Bosone C, Burkard TR, Spanier J, Kalinke U, Calistri A, Salata C, Rilo Christoff R, Pestana Garcez P, Mirazimi A, Knoblich JA. Cell Stem Cell. 2021 28(8):1362-1379.e7.

Central amygdala circuit dynamics underlying the benzodiazepine anxiolytic effect. Griessner J, Pasieka M, Böhm V, Grössl F, Kaczanowska J, Pliota P, Kargl D, Werner B, Kaouane N, Strobelt S, Kreitz S, Hess A, Haubensak W. Mol Psychiatry 2021 26(2):534-544.

RANK links thymic regulatory T cells to fetal loss and gestational diabetes in pregnancy. Paolino M, Koglgruber R, Cronin SJF, Uribesalgo I, Rauscher E, Harreiter J, Schuster M, Bancher-Todesca D, Pranjic B, Novatchkova M, Fededa JP, White AJ, Sigl V, Dekan S, Penz T, Bock C, Kenner L, Holländer GA, Anderson G, Kautzky-Willer A, Penninger JM. Nature. 2021 589(7842):442-447.

Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling. Fennell LM, Gomez Diaz C, Deszcz L, Kavirayani A, Hoffmann D, Yanagitani K, Schleiffer A, Mechtler K, Hagelkruys A, Penninger J, Ikeda F. [2020] EMBO J. 39(24):e103303

A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves. Nordmann, G.C., Malkemper, E.P., Landler, L. et al. [2020] Sci Rep 10, 915

The amygdala instructs insular feedback for affective learning. Kargl D, Kaczanowska J, Ulonska S, Groessl F, Piszczek L, Lazovic J, Buehler K, Haubensak W. [2020] Elife. 9:e60336.

A human tissue screen identifies a regulator of ER secretion as a brain-size determinant. Esk C, Lindenhofer D, Haendeler S, Wester RA, Pflug F, Schroeder B, Bagley JA, Elling U, Zuber J, von Haeseler A, Knoblich JA. [2020] Science. 370(6519):935-941.

A crucial role for Jagunal homolog 1 in humoral immunity and antibody glycosylation in mice and humans. Hagelkruys A, Wirnsberger G, Stadlmann J, Wöhner M, Horrer M, Vilagos B, Jonsson G, Kogler M, Tortola L, Novatchkova M, Bönelt P, Hoffmann D, Koglgruber R, Steffen U, Schett G, Busslinger M, Bergthaler A, Klein C, Penninger JM. [2020] J Exp Med. 218(1):e20200559.

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin. Borg M, Jacob Y, Susaki D, LeBlanc C, Buendía D, Axelsson E, Kawashima T, Voigt P, Boavida L, Becker J, Higashiyama T, Martienssen R, Berger F. [2020] Nat Cell Biol. 22(6):621-629

Chromatin Organization in Early Land Plants Reveals an Ancestral Association between H3K27me3, Transposons, and Constitutive Heterochromatin. Montgomery SA, Tanizawa Y, Galik B, Wang N, Ito T, Mochizuki T, Akimcheva S, Bowman JL, Cognat V, Maréchal-Drouard L, Ekker H, Hong SF, Kohchi T, Lin SS, Liu LD, Nakamura Y, Valeeva LR, Shakirov EV, Shippen DE, Wei WL, Yagura M, Yamaoka S, Yamato KT, Liu C, Berger F. [2020] Curr Biol. 30(4):573-588.e7.

Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4+CD25+FoxP3+ regulatory T cells activation. Lo Re O, Mazza T, Giallongo S, Sanna P, Rappa F, Vinh Luong T, Li Volti G, Drovakova A, Roskams T, Van Haele M, Tsochatzis E, Vinciguerra M. [2020] Theranostics 10(2):910-924.

Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection. Colaço HG, Barros A, Neves-Costa A, Seixas E, Pedroso D, Velho T, Willmann KL, Faisca P, Grabmann G, Yi HS, Shong M, Benes V, Weis S, Köcher T, Moita LF. [2020] Immunity. 54(1):53-67.e7

3D Printing of large-scale and highly porous biodegradable tissue engineering scaffolds from poly(trimethylene-carbonate) using two-photon-polymerization. Weisgrab G, Guillaume O, Guo Z, Heimel P, Slezak P, Poot A, Grijpma D, Ovsianikov A. [2020] Biofabrication. 12(4):045036.

Apelin inhibition prevents resistance and metastasis associated with anti‐angiogenic therapyUribesalgo I, Hoffmann D, Zhang Y, Kavirayani A, Lazovic J, Berta J, Novatchkova M, Pai TP, Wimmer RA, László V, Schramek D, Karim R, Tortola L, Deswal S, Haas L, Zuber J, Szűcs M, Kuba M., Dome B, Cao Y, Haubner BJ, Penninger JM. EMBO Mol Med. 2019 11(8)

HACE1 deficiency leads to structural and functional neurodevelopmental defects Nagy V, Hollstein R, Pai TP, Herde MK, Buphamalai P, Moeseneder P, Lenartowicz E, Kavirayani A, Korenke GC, Kozieradzki I, Nitsch R, Cicvaric A, Monje Quiroga FJ, Deardorff MA, Bedoukian EC, Li Y, Yigit G, Menche J, Perçin EF, Wollnik B, Henneberger C, Kaiser FJ, Penninger JM, Neurol Genet Jun 2019, 5 (3) e330

Cdk6 coordinates Jak2V617F mutant MPN via NFκB and apoptotic networks Uras IZ, Maurer B, Nivarthi H, Jodl P, Kollmann K, Prchal-Murphy M, Milosevic Feenstra JD, Zojer M, Lagger S, Grausenburger R, Grabner B, Holly R, Kavirayani A, Bock C, Gisslinger H, Valent P, Kralovics R, Sexl V, Blood. 2019 133(15)

Precocious expression of Blimp1 in B cells causes autoimmune disease with increased self-reactive plasma cells Bönelt P, Wöhner M, Minnich M, Tagoh H, Fischer M, Jaritz M, Kavirayani A, Garimella M, Karlsson MCI, Busslinger M. EMBO J.2019 38(2).

A simple and robust protocol for immunostaining Arabidopsis pollen nuclei. Borg M, Buendía D, Berger F, Plant Reproduction. 2019 doi.org/10.1007/s00497-018-00360-7

Human blood vessel organoids as a model of diabetic vasculopathy.Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, Taubenschmid J, Hämmerle M, Esk C, Bagley JA, Lindenhofer D, Chen G, Boehm M, Agu CA, Yang F, Fu B, Zuber J, Knoblich JA, Kerjaschki D, Penninger JM, Nature 2019 565(7740):505-510

Genetically engineered cerebral organoids model brain tumor formation. Bian S, Repic M, Guo Z, Kavirayani A, Burkard T, Bagley JA, Krauditsch C & Knoblich JA, Nat. Methods. 2018 Aug; 15(8): 631-639.

Central amygdala circuit dynamics underlying the benzodiazepine anxiolytic effect. Griessner J, Pasieka M, Böhm V, Grössl F, Kaczanowska J, Pliota P, Kargl D, Werner B, Kaouane N, Strobelt S, Kreitz S, Hess A, Haubensak W, Mol Psychiatry. 2018 Nov 30

Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Gerber T, Murawala P, Knapp D, Masselink W, Schuez M, Hermann S, Gac-Santel M, Nowoshilow S, Kageyama J, Khattak S, Currie JD, Camp JG, Tanaka EM, Treutlein B, Science, 2018 Oct; 362 (6413): eaaq0681

Dorsal tegmental dopamine neurons gate associative learning of fear. Groessl F, Munsch T, Meis S, Griessner J, Kaczanowska J, Pliota P, Kargl D, Badurek S, Kraitsy K, Rassoulpour A, Zuber J, Lessmann V, Haubensak W, Nat. Neurosci 2018 Jul;21(7):952-62

The axolotl genome and the evolution of key tissue formation regulators. Nowoshilow S, Schloissnig S, Fei JF, Dahl A, Pang AWC, Pippel M, Winkler S, Hastie AR, Young G, Roscito JG, Falcon F, Knapp D, Powell S, Cruz A, Cao H, Habermann B, Hiller M, Tanaka EM, Myers EW, Nature, 2018 Feb; 554: 50-55

Natural killer cell-intrinsic type I IFN signaling controls Klebsiella pneumoniae growth during lung infection. Ivin M, Dumigan A, de Vasconcelos FN, Ebner F, Borroni M, Kavirayani A, Przybyszewska KN, Ingram RJ, Lienenklaus S, Kalinke U, Stoiber D, Bengoechea JA, Kovarik P, PLoS Pathog. 2017 Nov; 13(11): e1006696.

RANK rewires energy homeostasis in lung cancer cells and drives primary lung cancer. Rao S, Sigl V, Wimmer RA, Novatchkova M, Jais A, Wagner G, Handschuh S, Uribesalgo I, Hagelkruys A, Kozieradzki I, Tortola L, Nitsch R, Cronin SJ, Orthofer M, Branstetter D, Canon J, Rossi J, D'Arcangelo M, Botling J, Micke P, Fleur L, Edlund K, Bergqvist M, EkmanS, Lendl T, Popper H, Takayanagi H, Kenner L, Hirsch FR, Dougall W, Penninger JM, Genes Dev. 2017 Oct; 31(20): 2099-2112

BET-Bromodomain Inhibitors Engage the Host Immune System and Regulate Expression of the Immune Checkpoint Ligand PD-L1. Hogg SJ, Vervoort SJ, Deswal S, Ott CJ, Li J, Cluse LA, Beavis PA, Darcy PK, Martin BP, Spencer A, Traunbauer AK, Sadovnik I, Bauer K, Valent P, Bradner JE, Zuber J, Shortt J, Johnstone RW, Cell Rep. 2017 Feb; 18(9): 2162-2174

Linear ubiquitination by LUBEL has a role in Drosophila heat stress response. Asaoka T, Almagro J, Ehrhardt C, Tsai I, Schleiffer A, Deszcz L, Junttila S, Ringrose L, Mechtler K, Kavirayani A, Gyenesei A, Hofmann K, Duchek P, Rittinger K, Ikeda F, EMBO Rep. 2016 Nov; 17(11): 1624-1640.

Inhibition of CBLB protects from lethal Candida albicans sepsis. Wirnsberger G, Zwolanek F, Asaoka T, Kozieradzki I, Tortola L, Wimmer RA, Kavirayani A, Fresser F, Baier G, Langdon WY, Ikeda F, Kuchler K, Penninger JM, Nat Med. 2016 Aug; 22(8): 915-923

RANKL/RANK control Brca1 mutation-driven mammary tumorsSigl V, Owusu-Boaitey K, Joshi PA, Kavirayani A, Wirnsberger G, Novatchkova M, Kozieradzki I, Schramek D, Edokobi N, Hersl J, Sampson A, Odai-Afotey A, Lazaro C, Gonzalez-Suarez E, Pujana MA, Cimba F, Heyn H, Vidal E, Cruickshank J, Berman H, Sarao R, Ticevic M, Uribesalgo I, Tortola L, Rao S, Tan Y, Pfeiler G, Lee EY, Bago-Horvath Z, Kenner L, Popper H, Singer C, Khokha R, Jones LP, Penninger JM, Cell Res. 2016 Jul; 26(7): 761-774.

Fast volumetric calcium imaging across multiple cortical layers using sculpted light.  Prevedel R, Verhoef AJ, Pernía-Andrade AJ, Weisenburger S, Huang BS, Nöbauer T, Fernández A, Delcour JE, Golshani P, Baltuska A, Vaziri A, Nat Methods, 2016 Dec; 13(12): 1021-1028