Immunohistochemical stains

The Histopathology facility offers chromogenic and fluorescent immunostaining for a variety of markers in diverse target tissues. Clicking on the links will take you to high resolution images corresponding to the representative examples listed below.

 

1. Nervous system markers

  • Neuronal nuclear antigen (RBFOX3/NeuN)
  • Glial fibrillary acidic protein (GFAP)
  • Tyrosine hydroxylase (TH)
  • Myelin basic protein (MBP) 

 

2. Immune / inflammatory cell markers

  • Forkhead box protein 3 (FOXP3)
  • CD3 antigen, epsilon (CD3e)
  • Protein tyrosine phosphatase receptor type C (B220/CD45R)
  • CD44 antigen (CD44)
  • Adhesion G protein coupled receptor E1 (ADGRE1/F4.80) 

 

3. Vascular and stromal markers

  • Platelet endothelial cell adhesion molecule 1 (CD31)
  • Collagen type IV (COL4) & Platelet endothelial cell adhesion molecule 1 (CD31]

 

4. Apoptosis marker

  • Caspase 3, cleaved (CASP3, cl)

 

5. Cellular proliferation markers

  • Antigen Ki-67 [Ki-67]
  • Histone H3, phosphorylated (PHH3)
  • Bromodeoxyuridine (BrdU) 

 

6. Histones and DNA damage markers

  • Histone 2A histone family, member X (gammaH2AX)
  • Histone 2A histone family, member Y, variant 1 (mH2A1.1)
  • Tumor protein 53 binding protein 1 (TP53BP1]

 

7. Epithelial, myoepithelial, basal, stem cell markers

  • Sex determining region Y box 1 (SOX1)
  • Transformation related protein 63 (TRP63)
  • Epithelial cell adhesion molecule (EPCAM)
  • Keratin 5 (KRT5)
  • Keratin 19 (KRT19)
  • Transformation related protein 63 (TRP63) & Keratin 14 (KRT14)

NERVOUS SYSTEM MARKERS

Neuronal nuclear antigen (NeuN)

Pyramidal neurons and dentate gyrus granular cells – brain (hippocampus), mouse

Glial fibrillary acidic protein (GFAP)

Astrocytes – brain (hippocampus), mouse

Tyrosine Hydroxylase (TH)

Dopaminergic neurons – brain (substantia nigra), mouse

Myelin basic protein (MBP)

Axons – sciatic nerve, mouse

IMMUNE / INFLAMMATORY CELL MARKERS

Forkhead box protein P3 (FOXP3)

Regulatory T lymphocytes – tonsil, human

CD3 antigen, epsilon (CD3e)

T lymphocytes – lymph node, human

Protein tyrosine phosphatase receptor type C (B220/CD45R)

B lymphocytes – spleen, mouse

CD44 antigen (CD44)

Medullary thymocytes – thymus, mouse

Adhesion G protein coupled receptor E1 (ADGRE1/F4.80)

Subcutaneous macrophages – skin, mouse

VASCULAR & STROMAL MARKERS

Platelet endothelial cell adhesion molecule 1 (CD31)

Vascular endothelium – stromal vessels, mammary tumor, mouse

Collagen type IV (COL4) & Platelet endothelial cell adhesion molecule 1 (CD31)

Pre-existent arterioles within tumor stroma – melanoma, mouse

APOPTOSIS MARKERS

Caspase 3, cleaved (CASP3cl)

Apoptotic epithelial cells – intestinal crypt, jejunum, mouse

Caspase 3, cleaved (CASP3cl)

Apoptotic lymphocytes – Peyer’s patch, jejunum, mouse

CELLULAR PROLIFERATION MARKERS

Antigen Ki-67 (Ki-67)

Outer root sheath epithelial nuclei, hair follicle – skin, mouse

Antigen Ki-67 (Ki-67)

Tumor cell nuclei – lung tumor, mouse

Histone H3, phosphorylated (PHH3)

Mitotic bodies, tumor cell nuclei – mammary tumor, mouse

Bromodeoxyuridine (BrdU)

Intestinal and pancreatic epithelial cell nuclei – duodenum, mouse

HISTONES AND DNA DAMAGE MARKERS

Histone 2A histone family, member X (gammaH2AX)

Neoplastic epithelial cell nuclei – mammary tumor, mouse

Histone 2A histone family, member Y, variant 1 (mH2A1.1)

Biliary epithelial nuclei – liver, human

Tumor protein 53 binding protein 1 (TP53BP1)

Tumor cell nuclei – mammary tumor, mouse

EPITHELIAL, MYOEPITHELIAL, BASAL, STEM CELL MARKERS

Sex determining region Y box 1 (SOX1)

Tumor cell nuclei – cerebral organoid tumor

Transformation related protein 63 (TRP63)

Urothelial nuclei, renal pelvis – kidney, mouse

Epithelial cell adhesion molecule (EPCAM)

Villous epithelial cytoplasmic membrane – duodenum, mouse

Keratin 5 (KRT5)

Tumor epithelial cytoplasm and membrane – mammary tumor, mouse

Keratin 19 (KRT19)

Tumor epithelial cytoplasm and membrane – lung tumor, mouse

Transformation related protein 63 (TRP63) & Keratin 14 (KRT14)

Epithelial cytoplasm and membrane (KRT14] and nuclei (TRP63], pilosebaceous units – skin, mouse