Next Generation Sequencing
Genomics’ most flexible and powerful tool to accelerate your research!
Next Generation Sequencing (NGS) has become a key analysis method for biological research, enabling researchers to obtain a global view of biological processes.
Advice and guidance for sequencing projects are offered by our team, which relies on more than 15 years of experience with sequencing systems, high-throughput data analysis, and cutting-edge NGS technology. All common sequencing applications are supported, and the development of novel methods and protocols is encouraged.
Contact us or visit our Info Hub
Library preparation
A prerequisite for NGS is the generation of sequencing libraries from the corresponding DNA or RNA samples. There are many protocols and kits available depending on the sequencing technology, sample source, and biological question of interest. The Next Generation Sequencing Core Facility offers a broad range of library preparation methods, including single-cell and long-read sequencing as well as spatial transcriptomics.
Quality control
There is a careful quality check of each sequencing library prepared by facility staff or users. This includes three major steps:
- Measurement of concentration (Fluorescence NanoDrop)
- Measurement of size (Bioanalyzer, Fragment Analyzer)
- Mimicking cluster formation (RT-PCR)
Sequencing
Depending on the application and biological question, sequencing is performed on Illumina (NovaSeq X, NextSeq 2000, MiSeq), Element Bio (Aviti), PacBio (Revio, in collaboration with the University of Bern), and ONT (MinION, PromethION) instruments. The available instruments cover short, long, and ultra-long sequencing and a broad range of output, from a few million reads to Terabases.
Data analysis
We generate quality metrics from the sequencing process and analyze the reads to ensure successful sequencing. Lanes running pools of multiple samples are demultiplexed by the index barcodes into one file per sample, and their quality is checked individually. On-demand, we offer mapping to reference genomes of model organisms. The alignment data can be visualized in available genome browsers and further analyzed in Galaxy, a community-driven web-based analysis platform for life science research. Standardized analysis pipelines are also available for many common applications (ChIP-seq, RNA-seq, SNP-mapping, small genome assembly).
Forskalle – For submission and administration of your sequencing requests.
Queue– A page to check the progress of your requests and securely download finished sequencing data.
Reserva – Book a time slot for the Covaris DNA shearer and MiSeq.
VBCF-NGS GitHub – Illumina-related tools and snippets for quality metrics and file conversion.
VBCF NEXT GENERATION SEQUENCING TEAM
SCIENTIFIC CONTRIBUTIONS
Inducible protein degradation reveals inflammation-dependent function of the Treg cell lineage–defining transcription factor Foxp3. Christina Jäger, Polina Dimitrova, Qiong Sun, Jesse Tennebroek, Elisa Marchiori, Markus Jaritz, Rene Rauschmeier, Guillem Estivill, Anna Obenauf, Meinrad Busslinger, Joris van der Veeken. 2025 Jun 6;10(108):eadr7057. doi: 10.1126/sciimmunol.adr7057. Epub 2025 Jun 6.
Intron-mediated induction of phenotypic heterogeneity. Lukačišin M, Espinosa-Cantú A, Bollenbach T. Nature. 2022 605(7908):113-118.
Differential cofactor dependencies define distinct types of human enhancers. Neumayr C, Haberle V, Serebreni L, Karner K, Hendy O, Boija A, Henninger JE, Li CH, Stejskal K, Lin G, Bergauer K, Pagani M, Rath M, Mechtler K, Arnold CD, Stark A. Nature. 2022 606(7913):406-413.
Amplification of human interneuron progenitors promotes brain tumors and neurological defects. Eichmüller OL, Corsini NS, Vértesy Á, Morassut I, Scholl T, Gruber VE, Peer AM, Chu J, Novatchkova M, Hainfellner JA, Paredes MF, Feucht M, Knoblich JA. Science. 2022 375(6579):eabf5546.
Zygotic genome activation by the totipotency pioneer factor Nr5a2. Gassler J, Kobayashi W, Gáspár I, Ruangroengkulrith S, Mohanan A, Gómez Hernández L, Kravchenko P, Kümmecke M, Lalic A, Rifel N, Ashburn RJ, Zaczek M, Vallot A, Cuenca Rico L, Ladstätter S, Tachibana K. Science. 2022 378(6626):1305-1315
For the full list of scientific contributions, click here.
Long-term gut microbiome dynamics in Drosophila melanogaster reveal environment-specific associations between bacterial taxa at the family level. Mazzucco R, Schlötterer C. Proc Biol Sci. 2021 288(1965):20212193.
Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. Bajaj S, Bagley JA, Sommer C, Vertesy A, Nagumo Wong S, Krenn V, Lévi-Strauss J, Knoblich JA. EMBO J. 2021 40(23):e108714.
An inhibitor-mediated beta-cell dedifferentiation model reveals distinct roles for FoxO1 in glucagon repression and insulin maturation. Casteels T, Zhang Y, Frogne T, Sturtzel C, Lardeau CH, Sen I, Liu X, Hong S, Pauler FM, Penz T, Brandstetter M, Barbieux C, Berishvili E, Heuser T, Bock C, Riedel CG, Meyer D, Distel M, Hecksher-Sørensen J, Li J, Kubicek S. Mol Metab. 2021 54:101329.
Anammox bacteria drive fixed nitrogen loss in hadal trench sediments. Thamdrup B, Schauberger C, Larsen M, Trouche B, Maignien L, Arnaud-Haond S, Wenzhöfer F, Glud RN. Proc Natl Acad Sci U S A. 2021 118(46):e2104529118.
Highly Parallel Genomic Selection Response in Replicated Drosophila melanogaster Populations with Reduced Genetic Variation. Burny C, Nolte V, Dolezal M, Schlötterer C. Genome Biol Evol. 2021 13(11):evab239.
PhosphoFlowSeq - A High-throughput Kinase Activity Assay for Screening Drug Resistance Mutations in EGFR. Wagner A, Teufl M, Gold L, Lehner M, Obinger C, Sykacek P, Traxlmayr MW. J Mol Biol. 2021 433(22):167210.
Autochthonous fungi are central components in microbial community structure in raw fermented sausages. Zwirzitz B, Thalguter S, Wetzels SU, Stessl B, Wagner M, Selberherr E. Microb Biotechnol. 2021, doi: 10.1111/1751-7915.13950. Epub ahead of print.
PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. Appel LM, Franke V, Bruno M, Grishkovskaya I, Kasiliauskaite A, Kaufmann T, Schoeberl UE, Puchinger MG, Kostrhon S, Ebenwaldner C, Sebesta M, Beltzung E, Mechtler K, Lin G, Vlasova A, Leeb M, Pavri R, Stark A, Akalin A, Stefl R, Bernecky C, Djinovic-Carugo K, Slade D. Nat Commun. 2021 12(1):6078.
miR-1 sustains muscle physiology by controlling V-ATPase complex assembly. Gutiérrez-Pérez P, Santillán EM, Lendl T, Wang J, Schrempf A, Steinacker TL, Asparuhova M, Brandstetter M, Haselbach D, Cochella L. Sci Adv. 2021 7(42):eabh1434.
The Evolutionary History of New Zealand Deschampsia Is Marked by Long-Distance Dispersal, Xue Z, Greimler J, Paun O, Ford KA, Barfuss MHJ, Chiapella JO. Endemism, and Hybridization. Biology (Basel). 2021 10(10):1001.
Gradual evolution of allopolyploidy in Arabidopsis suecica. Burns R, Mandáková T, Gunis J, Soto-Jiménez LM, Liu C, Lysak MA, Novikova PY, Nordborg M. Nat Ecol Evol. 2021 5(10):1367-1381.
Handling of targeted amplicon sequencing data focusing on index hopping and demultiplexing using a nested metabarcoding approach in ecology. Guenay-Greunke Y, Bohan DA, Traugott M, Wallinger C. Sci Rep. 2021 11(1):19510.
Reprogrammed lipid metabolism protects inner nuclear membrane against unsaturated fat. Romanauska A, Köhler A. Dev Cell. 2021 56(18):2562-2578.e3.
Comparative analysis reveals within-population genome size variation in a rotifer is driven by large genomic elements with highly abundant satellite DNA repeat elements. Stelzer CP, Blommaert J, Waldvogel AM, Pichler M, Hecox-Lea B, Mark Welch DB. BMC Biol. 2021 19(1):206.
Copy Number Changes and Allele Distribution Patterns of Chromosome 21 in B Cell Precursor Acute Lymphoblastic Leukemia. Abbasi MR, Nebral K, Haslinger S, Inthal A, Zeitlhofer P, König M, Schinnerl D, Köhrer S, Strehl S, Panzer-Grümayer R, Mann G, Attarbaschi A, Haas OA. Cancers (Basel). 2021 13(18):4597.
A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. Capitao C, Tanasa S, Fulnecek J, Raxwal VK, Akimcheva S, Bulankova P, Mikulkova P, Crhak Khaitova L, Kalidass M, Lermontova I, Mittelsten Scheid O, Riha K. PLoS Genet. 2021 17(9):e1009779.
A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells. Schmieder V, Novak N, Dhiman H, Nguyen LN, Serafimova E, Klanert G, Baumann M, Kildegaard HF, Borth N. Biotechnol Rep (Amst). 2021 31:e00649.
Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants. Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA, Darino M, Pĕnčík A, Novák O, Kalyna M, Mouille G, Benková E, P Bhalerao R, Mravec J, Kleine-Vehn J. Int J Mol Sci. 2021 22(17):9222.
Quinoa genome assembly employing genomic variation for guided scaffolding. Bodrug-Schepers A, Stralis-Pavese N, Buerstmayr H, Dohm JC, Himmelbauer H. Theor Appl Genet. 2021 134(11):3577-3594.
LINC01133 Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line. Yotova I, Hudson QJ, Pauler FM, Proestling K, Haslinger I, Kuessel L, Perricos A, Husslein H, Wenzl R. Int J Mol Sci. 2021 22(16):8385.
Ecdysis-related neuropeptide expression and metamorphosis in a non-ecdysozoan bilaterian. Zieger E, Calcino AD, Robert NSM, Baranyi C, Wanninger A. Evolution. 2021 75(9):2237-2250.
Repression of CHROMOMETHYLASE 3 prevents epigenetic collateral damage in Arabidopsis. Papareddy RK, Páldi K, Smolka AD, Hüther P, Becker C, Nodine MD. Elife. 2021 10:e69396.
Pax5 regulates B cell immunity by promoting PI3K signaling via PTEN down-regulation. Calderón L, Schindler K, Malin SG, Schebesta A, Sun Q, Schwickert T, Alberti C, Fischer M, Jaritz M, Tagoh H, Ebert A, Minnich M, Liston A, Cochella L, Busslinger M. Sci Immunol. 2021 6(61):eabg5003.
The genetic architecture of temperature adaptation is shaped by population ancestry and not by selection regime. Otte KA, Nolte V, Mallard F, Schlötterer C. Genome Biol. 2021 22(1):211.
Expression of RUNX1-JAK2 in Human Induced Pluripotent Stem Cell-Derived Hematopoietic Cells Activates the JAK-STAT and MYC Pathways. Fortschegger K, Husa AM, Schinnerl D, Nebral K, Strehl S. Int J Mol Sci. 2021 22(14):7576.
Transcriptomics unravels molecular players shaping dorsal lip hypertrophy in the vacuum cleaner cichlid, Gnathochromis permaxillaris. Lecaudey LA, Singh P, Sturmbauer C, Duenser A, Gessl W, Ahi EP. BMC Genomics. 2021 22(1):506.
Gene expression variation in Arabidopsis embryos at single-nucleus resolution. Kao P, Schon MA, Mosiolek M, Enugutti B, Nodine MD. Development. 2021 148(13):dev199589.
Ovulation suppression protects against chromosomal abnormalities in mouse eggs at advanced maternal age. Chatzidaki EE, Powell S, Dequeker BJH, Gassler J, Silva MCC, Tachibana K. Curr Biol. 2021 31(18):4038-4051.e7.
The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Jenull S, Mair T, Tscherner M, Penninger P, Zwolanek F, Silao FS, de San Vicente KM, Riedelberger M, Bandari NC, Shivarathri R, Petryshyn A, Chauhan N, Zacchi LF, -Landmann SL, Ljungdahl PO, Kuchler K. Cell Rep. 2021 36(3):109406.
Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Marx N, Dhiman H, Schmieder V, Freire CM, Nguyen LN, Klanert G, Borth N. Metab Eng. 2021 66:268-282.
Genomic insights into diverse bacterial taxa that degrade extracellular DNA in marine sediments. Wasmund K, Pelikan C, Schintlmeister A, Wagner M, Watzka M, Richter A, Bhatnagar S, Noel A, Hubert CRJ, Rattei T, Hofmann T, Hausmann B, Herbold CW, Loy A. Nat Microbiol. 2021 6(7):885-898.
Bacteria of eleven different species isolated from biofilms in a meat processing environment have diverse biofilm forming abilities. Wagner EM, Fischel K, Rammer N, Beer C, Palmetzhofer AL, Conrady B, Roch FF, Hanson BT, Wagner M, Rychli K. Int J Food Microbiol. 2021 349:109232.
Favorable outcome of NUTM1-rearranged infant and pediatric B cell precursor acute lymphoblastic leukemia in a collaborative international study. Boer JM, Valsecchi MG, Hormann FM, Antić Ž, Zaliova M, Schwab C, Cazzaniga G, Arfeuille C, Cavé H, Attarbaschi A, Strehl S, Escherich G, Imamura T, Ohki K, Grüber TA, Sutton R, Pastorczak A, Lammens T, Lambert F, Li CK, Carrillo de Santa Pau E, Hoffmann S, Möricke A, Harrison CJ, Den Boer ML, De Lorenzo P, Stam RW, Bergmann AK, Pieters R. Leukemia. 2021 35(10):2978-2982.
Soil fertility relates to fungal-mediated decomposition and organic matter turnover in a temperate mountain forest. Mayer M, Rewald B, Matthews B, Sandén H, Rosinger C, Katzensteiner K, Gorfer M, Berger H, Tallian C, Berger TW, Godbold DL. New Phytol. 2021 231(2):777-790.
A non-viral and selection-free COL7A1 HDR approach with improved safety profile for dystrophic epidermolysis bullosa. Kocher T, Bischof J, Haas SA, March OP, Liemberger B, Hainzl S, Illmer J, Hoog A, Muigg K, Binder HM, Klausegger A, Strunk D, Bauer JW, Cathomen T, Koller U. Mol Ther Nucleic Acids. 2021 25:237-250.
Uncovering Key Metabolic Determinants of the Drug Interactions Between Trimethoprim and Erythromycin in Escherichia coli. Qi Q, Angermayr SA, Bollenbach T. Front Microbiol. 2021 12:760017.
Antagonistic activities of cotranscriptional regulators within an early developmental window set FLC expression level. Schon M, Baxter C, Xu C, Enugutti B, Nodine MD, Dean C. Proc Natl Acad Sci U S A. 2021 118(17):e2102753118.
Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces. Paredes GF, Viehboeck T, Lee R, Palatinszky M, Mausz MA, Reipert S, Schintlmeister A, Maier A, Volland JM, Hirschfeld C, Wagner M, Berry D, Markert S, Bulgheresi S, König L. mSystems. 2021 6(3):e0118620
Genomic imprinting in mouse blastocysts is predominantly associated with H3K27me3. Santini L, Halbritter F, Titz-Teixeira F, Suzuki T, Asami M, Ma X, Ramesmayer J, Lackner A, Warr N, Pauler F, Hippenmeyer S, Laue E, Farlik M, Bock C, Beyer A, Perry ACF, Leeb M. Nat Commun. 2021 12(1):3804
Central amygdala circuitry modulates nociceptive processing through differential hierarchical interaction with affective network dynamics. Wank, I., Pliota, P., Badurek, S., Kraitsy, K., Kaczanowska J., Griessner J., Kreitz S., Hess A., Haubensak W. Commun Biol 2021 4, 732.
Cardioids reveal self-organizing principles of human cardiogenesis. Hofbauer P, Jahnel SM, Papai N, Giesshammer M, Deyett A, Schmidt C, Penc M, Tavernini K, Grdseloff N, Meledeth C, Ginistrelli LC, Ctortecka C, Salic S, Novatchkova M, Mendjan S (2021) Cell 184 (12) 3299-3317.e22.
Subcloning induces changes in the DNA-methylation pattern of outgrowing Chinese hamster ovary cell colonies. Weinguny M, Klanert G, Eisenhut P, Lee I, Timp W, Borth N. Biotechnol J. 2021 16(6):e2000350.
Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq. Yelagandula R, Bykov A, Vogt A, Heinen R, Özkan E, Strobl MM, Baar JC, Uzunova K, Hajdusits B, Kordic D, Suljic E, Kurtovic-Kozaric A, Izetbegovic S, Schaeffer J, Hufnagl P, Zoufaly A, Seitz T; VCDI, Födinger M, Allerberger F, Stark A, Cochella L, Elling U. Nat Commun. 2021 12(1):3132
Fibroblast dedifferentiation as a determinant of successful regeneration. Lin TY, Gerber T, Taniguchi-Sugiura Y, Murawala P, Hermann S, Grosser L, Shibata E, Treutlein B, Tanaka EM. Dev Cell. 2021 56(10):1541-1551.
Selective requirement of MYB for oncogenic hyperactivation of a translocated enhancer in leukemia. Smeenk L, Ottema S, Mulet-Lazaro R, Ebert A, Havermans M, Arricibita Varea A, Fellner M, Pastoors D, van Herk S, Erpelinck-Verschueren C, Grob T, Hoogenboezem RM, Kavelaars FG, Matson DR, Bresnick EH, Bindels EM, Kentsis A, Zuber J, Delwel R. Cancer Discov. 2021 doi: 10.1158/2159-8290.CD-20-1793. Epub ahead of print.
A tale of two morphs: developmental patterns and mechanisms of seed coat differentiation in the dimorphic diaspore model Aethionema arabicum (Brassicaceae). Arshad W, Lenser T, Wilhelmsson PKI, Chandler JO, Steinbrecher T, Marone F, Pérez M, Collinson ME, Stuppy W, Rensing SA, Theißen G, Leubner-Metzger G. Plant J. 2021 107(1):166-181
The giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci. Schloissnig S, Kawaguchi A, Nowoshilow S, Falcon F, Otsuki L, Tardivo P, Timoshevskaya N, Keinath MC, Smith JJ, Voss SR, Tanaka EM. Proc Natl Acad Sci U S A. 2021 118(15):e2017176118
Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research. Fernandez-Pozo N, Metz T, Chandler JO, Gramzow L, Mérai Z, Maumus F, Mittelsten Scheid O, Theißen G, Schranz ME, Leubner-Metzger G, Rensing SA. Plant J. 2021 106(1):275-293
The chromatin remodeler DDM1 prevents transposon mobility through deposition of histone variant H2A.W. Osakabe A, Jamge B, Axelsson E, Montgomery SA, Akimcheva S, Kuehn AL, Pisupati R, Lorković ZJ, Yelagandula R, Kakutani T, Berger F. Nat Cell Biol. 2021 23(4):391-400.
Data of de novo transcriptome assembly of the myxozoan parasite Tetracapsuloides bryosalmonae. Kumar G, Ertl R, Nilsen F, Bartholomew JL, El-Matbouli M. Data Brief. 2021 35:106831
Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly. Krenn V, Bosone C, Burkard TR, Spanier J, Kalinke U, Calistri A, Salata C, Rilo Christoff R, Pestana Garcez P, Mirazimi A, Knoblich JA. Cell Stem Cell. 2021 28(8):1362-1379.e7.
High Fungal Diversity but Low Seasonal Dynamics and Ectomycorrhizal Abundance in a Mountain Beech Forest. Gorfer M, Mayer M, Berger H, Rewald B, Tallian C, Matthews B, Sandén H, Katzensteiner K, Godbold DL. Microb Ecol. 2021 82(1):243-256.
Comparing de novo transcriptome assembly tools in di- and autotetraploid non-model plant species. Madritsch S, Burg A, Sehr EM. BMC Bioinformatics. 2021 22(1):146
Temporal dissection of an enhancer cluster reveals distinct temporal and functional contributions of individual elements. Thomas HF, Kotova E, Jayaram S, Pilz A, Romeike M, Lackner A, Penz T, Bock C, Leeb M, Halbritter F, Wysocka J, Buecker C. Mol Cell. 2021 81(5):969-982
Polyploidy-associated paramutation in Arabidopsis is determined by small RNAs, temperature, and allele structure. Bente H, Foerster AM, Lettner N, Mittelsten Scheid O. PLoS Genet. 2021 17(3):e1009444.
Molecular principles of Piwi-mediated cotranscriptional silencing through the dimeric SFiNX complex. Schnabl J, Wang J, Hohmann U, Gehre M, Batki J, Andreev VI, Purkhauser K, Fasching N, Duchek P, Novatchkova M, Mechtler K, Plaschka C, Patel DJ, Brennecke J. Genes Dev. 2021 35(5-6):392-409
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment. Pelikan C, Wasmund K, Glombitza C, Hausmann B, Herbold CW, Flieder M, Loy A. ISME J. 2021 15(3):833-847
Identification of gene targets of mutant C/EBPα reveals a critical role for MSI2 in CEBPA-mutated AML. Heyes E, Schmidt L, Manhart G, Eder T, Proietti L, Grebien F. Leukemia. 2021 doi: 10.1038/s41375-021-01169-6. Epub ahead of print.
ATM controls meiotic DNA double-strand break formation and recombination and affects synaptonemal complex organization in plants. Kurzbauer MT, Janisiw MP, Paulin LF, Prusén Mota I, Tomanov K, Krsicka O, Haeseler AV, Schubert V, Schlögelhofer P. Plant Cell. 2021 33(5):1633-1656
Biomolecular condensation of NUP98 fusion proteins drives leukemogenic gene expression.Terlecki-Zaniewicz S, Humer T, Eder T, Schmoellerl J, Heyes E, Manhart G, Kuchynka N, Parapatics K, Liberante FG, Müller AC, Tomazou EM, Grebien F. Nat Struct Mol Biol. 2021 28(2):190-201
Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition. Cabrera-Quio LE, Schleiffer A, Mechtler K, Pauli A. PLoS Genet. 2021 17(2):e1009390.
Central amygdala circuit dynamics underlying the benzodiazepine anxiolytic effect. Griessner J, Pasieka M, Böhm V, Grössl F, Kaczanowska J, Pliota P, Kargl D, Werner B, Kaouane N, Strobelt S, Kreitz S, Hess A, Haubensak W. Mol Psychiatry 2021 26(2):534-544.
Novel Identified Circular Transcript of RCAN2, circ-RCAN2, Shows Deviated Expression Pattern in Pig Reperfused Infarcted Myocardium and Hypoxic Porcine Cardiac Progenitor Cells In Vitro. Mester-Tonczar J, Einzinger P, Winkler J, Kastner N, Spannbauer A, Zlabinger K, Traxler D, Lukovic D, Hasimbegovic E, Goliasch G, Pavo N, Gyöngyösi M. Int J Mol Sci. 2021 22(3):1390
Epigenetic reprogramming rewires transcription during the alternation of generations in Arabidopsis. Borg M, Papareddy RK, Dombey R, Axelsson E, Nodine MD, Twell D, Berger F. Elife. 2021 10:e61894
Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants. Sims J, Sestini G, Elgert C, von Haeseler A, Schlögelhofer P. Nat Commun. 2021 12(1):387
Spatial and Ecological Drivers of Genetic Structure in Greek Populations of Alkanna tinctoria (Boraginaceae), a Polyploid Medicinal Herb. Ahmad M, Leroy T, Krigas N, Temsch EM, Weiss-Schneeweiss H, Lexer C, Sehr EM, Paun O. Front Plant Sci. 2021 12:706574
Ultra-High-Density QTL Marker Mapping for Seedling Photomorphogenesis Mediating Arabidopsis Establishment in Southern Patagonia. Matsusaka D, Filiault D, Sanchez DH, Botto JF. Front Plant Sci. 2021 12:677728
Evidence for Glacial Refugia of the Forest Understorey Species Helleborus niger (Ranunculaceae) in the Southern as Well as in the Northern Limestone Alps. Záveská E, Kirschner P, Frajman B, Wessely J, Willner W, Gattringer A, Hülber K, Lazić D, Dobeš C, Schönswetter P. Front Plant Sci. 2021 12:683043
Gene Expression Profiling of Pseudomonas aeruginosa Upon Exposure to Colistin and Tobramycin. Cianciulli Sesso A, Lilić B, Amman F, Wolfinger MT, Sonnleitner E, Bläsi U. Front Microbiol. 2021 12:626715
Co-Occurrence of Listeria spp. and Spoilage Associated Microbiota During Meat Processing Due to Cross-Contamination Events. Zwirzitz B, Wetzels SU, Dixon ED, Fleischmann S, Selberherr E, Thalguter S, Quijada NM, Dzieciol M, Wagner M, Stessl B. Front Microbiol. 2021 12:632935
TMT-Opsins differentially modulate medaka brain function in a context-dependent manner. Fontinha BM, Zekoll T, Al-Rawi M, Gallach M, Reithofer F, Barker AJ, Hofbauer M, Fischer RM, von Haeseler A, Baier H, Tessmar-Raible K. PLoS Biol. 2021 19(1):e3001012
Systematic refinement of gene annotations by parsing mRNA 3' end sequencing datasets. Bhat P, Burkard TR, Herzog VA, Pauli A, Ameres SL. Methods Enzymol. 2021;655:205-223
A crucial role for Jagunal homolog 1 in humoral immunity and antibody glycosylation in mice and humans. Hagelkruys A, Wirnsberger G, Stadlmann J, Wöhner M, Horrer M, Vilagos B, Jonsson G, Kogler M, Tortola L, Novatchkova M, Bönelt P, Hoffmann D, Koglgruber R, Steffen U, Schett G, Busslinger M, Bergthaler A, Klein C, Penninger JM. J Exp Med. 2021 218(1):e20200559.
Synthetic Approach to Reconstruct the Evolutionary and Functional Innovations of the Plant Histone Variant H2A.W. Lei B, Capella M, Montgomery SA, Borg M, Osakabe A, Goiser M, Muhammad A, Braun S, Berger F. A. Curr Biol. 2021 31(1):182-191.e5.