Plant Sciences

Take your plant research to the next level!

 

The PlantS facility has two focal points:

1. With 22 state-of-the-art plant growth chambers, we can precisely control various environmental conditions of different climate zones and reproduce all kinds of abiotic stress conditions.

2. We offer automated plant phenotyping for high-throughput assessment of plant phenotypic traits. The image analysis software can also be used for custom analysis of external images.

In 2017, PlantS initiated the Austrian Plant Phenotyping Network (APPN).

SERVICES

High-throughput plant phenotyping

For objective, reproducible, and high-throughput assessment of plant phenotypic traits, we operate an automated, chamber integrated, non-destructive screening system linked to LemnaTec image analysis software. The phenotyping platform (PHENOTron) is designed for top-view RGB imaging of small plants like Arabidopsis thaliana and is fully integrated into one of our high-tech phytotrons.

This particular phytotron facilitates frost stress experiments (> -15°C) and is equipped with a high-tech LED light with adjustable light spectrum.

We offer a complete phenotyping service: from seeds to data analysis!

High-throughput image analysis

The LemnaTec image analysis software is also used for custom analysis of externally acquired images e.g., agar plate-based screening of seedlings, plant roots, the side-view phenotyping of large plants, and for analysis of microscopically-derived images.

Environmental simulation

Several growth chambers can provide exceptional environmental conditions like low temperature (≥ -15°C), high temperature (≤ +50°C), different light intensities and light spectra (LED-light: blue_405, blue_450, white_warm, and red_660_730), and different gas conditions (e.g., CO2 atmosphere). This allows precise environmental simulation across different climate zones and of various environmental stress conditions.

The following plant stress conditions can be created in highly controlled environments:

  • Cold stress (≥ -15°C) 
  • Heat stress (≤ +50°C)
  • Light stress
  • Water stress (drought and partial waterlogging)

Basic plant growth service

Our growth chambers provide standard growth conditions with only minor deviations from the set points, independent of external environmental parameters. Significant deviations will be reported automatically 24/7. Temperature, humidity, light, and water supply are computer controlled and fully automated, and corresponding parameters are continuously graphed and documented.

Additionally, the facility offers premises for soil preparation and plant handling, equipped with microscopes, a laminar flow hood, and much more. Two experienced technicians take care of the plants including aspects of pest monitoring and pest control.

EQUIPMENT

Plant growth chamber

The PlatS facility operates 22 state-of-the-art plant growth chambers. For technical details please click HERE.

Specialized growth chambers:

The phenotyping platform PHENOTron

The phenotyping platform (PHENOTron) is designed for top-view RGB imaging of small plants like Arabidopsis thaliana and is fully integrated into one of our high-tech phytotrons.

This particular phytotron facilitates frost stress experiments (> -15°C) and is equipped with a high-tech LED light with adjustable light spectrum.

Side-view phenotyping platform for tall plants

A system for phenotyping of individual tall plants, e.g. model grass Brachypodium distachyon or maize. Coupled with PhenoPipe module for calculation of multi-dimensional distances from phenotyping data, we provide an affordable, automated, open source imaging and data processing solution that can be adapted to various phenotyping applications in plant biology and beyond.

Read more

Portable Fluorometer FluorPen FP 100-MAX-D (PSI)

FluorPen is a portable, battery-powered fluorometer that enables quick and precise measurement of chlorophyll fluorescence parameters in the laboratory, greenhouse, or in the field. It can be effectively used for studying photosynthetic activity, stress detection, herbicide testing, or mutant screening.

For more parameters click HERE.

USER INFORMATION

In case of any questions on our services/equipment and for project requests please contact the Facility Head Jakub Jez.

Project requests:

For detailed information on our services and specific project requests please contact Jakub Jez.

 

Training & general plant rules:

All new facility users must attend a training session on general plant rules. Please contact Mrs. Anneliese Auer. The General Plant Rules can be found in MyVBCF for download.

 

Need immediate assistance?

In case of technical problems, pest infestations, or other urgent issues please always write to: plants(at)vbcf.ac.at. The pest management guidelines can be found in MyVBCF for download.

 

Booking system

The continuation of funding for VBCF by the City of Vienna and Austrian Federal Ministry of Education, Science and Research is dependent on documented evidence of contributions to scientific output. Therefore, it is mandatory to acknowledge use of the facility when publishing work for which VBCF services were used.

A simple statement is sufficient and can either be placed in the Materials and Methods section or in the Acknowledgements.

 

Suggested format:

The XXXXXX was performed by the Plant Sciences Facility at Vienna BioCenter Core Facilities GmbH (VBCF), member of the Vienna BioCenter (VBC), Austria. 

 

In case of (co-)authorship:

The Vienna BioCenter Core Facilities GmbH (VBCF) Plant Sciences Facility acknowledges funding from the Austrian Federal Ministry of Education, Science and Research and the City of Vienna. (Your publication will then be listed on our website.)

PUBLICATIONS

The'PhenoBox', a flexible, automated, open-source plant phenotyping solution
Czedik-Eysenberg A., Seitner S., Güldener U., Koemeda S., Jez J., Colombini M., & Djamei A. The New phytologist, 2018.