Organisms and the tissues that constitute them, are fundamentally affected by a variety of intrinsic aberrations (e.g. gene dysfunctions) and extrinsic insults (e.g. infections). Histopathology enables insightful evaluation of the morphological manifestations of functional disturbances (e.g. aberrant gene expression).
The HistoPathology (HP) facility provides unique, combined expertise in histological techniques and comparative pathology to provide comprehensive tissue analyses. Our services encompass tissue processing, routine histological stains, a growing array of immunostaining techniques and in situ hybridization methods, and are delivered in highly individualized approaches for each project.
Do you work with tissue systems or animal models? Do you study disease processes? Are you interested in our services?
We can assist with study design, and development and implementation of protocols for standard as well as customized experimental studies.
We routinely provide services and support for:
- Sample collection, fixation, and processing
- Cryo- and paraffin embedding and sectioning
We have experience working with several types of samples including whole organisms, isolated organs, slices or groups of tissues, cell cultures (2D and 3D), fluids, and inorganic materials.
We perform routine and special histochemical stains along with immunostainings (immunofluorescence, immunohistochemistry, and multiplex stains). We have experience with background reduction in problematic “mouse on mouse” stainings as well as cross-reactivity avoidance during multiplex stainings. We also offer RNA in situ hybridization with basic protocols and RNAscope.
Our facility features in-house comparative pathology expertise for the evaluation of processing outcomes, analysis of morphological features and lesional parameters, and integration of the results of processing and analysis into manuscripts, presentations, or other pertinent documents. Pathology services offered by the VBCF HistoPathology facility include:
- Consultations and reviews
- Microscopic analyses of morphological features
- Representative imaging and documentation
- Digital quantification (in collaboration with IMP Biooptics facility)
- Manuscript support
Support during self-service is offered exclusively to IMP/IMBA/GMI members. It includes training in the use of all the sectioning equipment available in the facility and troubleshooting support.
Secondary and inter-facility support includes coordinated processing services (e.g., perfusion and dissection after in vivo imaging) offered with other facilities for mutilmodal projects. For example, HistoPathology staff work collaboratively with the IMP Biooptics core facility to facilitate whole slide digitization (slide scanning).
Correlative interpretation and validation of histomorphologic findings in relation to information obtained from other modalities is offered as part of pathology services.
A fully automated immunostainer with the capacity for 30 slides per run. It can perform immunochemistry staining with either DAB or AP-red detection systems in as little as 3 h and allows for high reproducibility.
If you are interested in having immunostains done on your samples with the Leica Bond III, please contact us.
This instrument performs automatic tissue dehydration, clearing, and paraffin infiltration. The LOGOS dehydrator competes in quality, versatility, and safety with other dehydrators on the market. It can perform 210 tissue blocks per run, with any kind of tissue up to 6 mm in thickness.
If you are interested in using the LOGOS, please contact us.
Microtome to section frozen or cryo-embedded tissues. Open-top cryostat with ergonomic design for working while standing or sitting. This cryostat allows a section thickness range from 0.5 to 100 µm. The temperature for the specimen clumper is controlled and ranges from 10°C to -35°C. There is a separate temperature control for the knife carrier down to -35°C.
Heavy duty microtomes used for paraffin sectioning, allowing a section thickness range from 0.5 to 100 µm. The sections can then be transferred onto a glass slide and used for a wide variety of staining protocols (basic staining, immunohistochemistry, in situ hybridization, or immunofluorescence).
To order a service, please contact the facility staff as early as possible to discuss relevant details and agree upon timelines. When submitting samples, please remember to label each sample with a unique identifier (e.g., mouse ID or organoid batch number), submitter initials, and date. Please also provide relevant details such as: genotype, treatment, or other procedure.If procedures relevant to coordinated processing have been performed (e.g. in vivo imaging) please ensure that pertinent details and representative images are provided. The clearer and more complete the information provided, the lower the risk of downstream errors. The facility can also provide you with submission forms for your projects.
IMP, IMBA, and GMI researchers can, by themselves, use all sectioning equipment in the facility themselves, but only after attending a training session with our staff. To set up an appointment for training, please contact the facility staff .
In addition, the facility offers many for-fee-services to members of the Vienna BioCenter (academic institutions and companies) as well as external academic institutions and private companies.
For paid service projects, the the users from the Vienna BioCenter have priority. Otherwise, the service projects are performed in the order they are requested; ‘first come, first served’.
Acknowledging the facility in publications and documents that feature results obtained through work performed in the facility is obligatory. Acknowledgments serve as a major indicator of the facility’s value to the research community and provide a basis to secure sustainable funding. They also encourage further provision of the highest quality services.
- “The service” was performed by the VBCF HistoPathology Facility (www.viennabiocenter.org/facilities).
- We thank the VBCF HistoPathology facility for (names of relevant methods and services).
- We thank (name of relevant facility staff members), VBCF HistoPathology facility for (names of relevant methods and services).
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