Advanced Microscopy

IMPORTANT: Advanced Microscopy services at Covid-19 Re-Entry operation

For detailed information on the current facility operations, please click here.

Microscopes that let you see things no one has ever seen before!

 

Optical microscopy has historically played an important role in life-science discovery. Advances in imaging technology are rapidly expanding the boundaries of what is possible, allowing for studies of live cells with unprecedented spatio-temporal resolution and the extraction of diverse biophysical parameters intrinsic to the processes of life. 

The Advanced Microscopy Facility offers a range of cutting-edge, novel and custom optical microscopy techniques, along with associated expertise and assistance, for life science researchers.

Interested in using one of our techniques? Need additional information? Got a specific project question?

SERVICES

Assisted operation of facility instruments & associated data analysis

Following discussion and planning of your project, expert facility staff perform the agreed experiments together with you and assist you with data analysis and interpretation as required for your project. An hourly fee per staff-hour and instrument-hour is charged.

Training on instrument for self-operation (on selected instruments)

For longer-term projects or if you are planning to use an instrument extensively/for several different projects, it may often be desirable to operate the instrument independent of facility staff availability. In this case, training on an instrument by an expert staff member is possibleTraining times will depend on the technique and instrument (estimates provided beforehand). Independent usage on completion of training at trainers’ discretion.

Independent operation of instruments

Instruments may be reserved via our online booking system. We currently have a 24 hr cancellation policy, after which the cost of the respective booked instrument-hours will be charged. Independent operation is charged at an instrument dependent hourly rate.

Consulting (experimental planning/design)

Consulting is provided free of charge if it is tied to the usage or potential usage of an instrument/technique at the facility. General consulting on microscopy related topics is provided at an hourly rate subject to staff availability.

EQUIPMENT

FLIM/FCS microscope

Time-domain FLIM, FLCS, FLCCS, line/imaging FCS, Fluorescence Anisotropy, etc.

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L-SPIM

Macro-SPIM, Meso-SPIM, Lattice Light Sheet.

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Confocal Brillouin and Raman scattering.

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Axi-TIRF Microscope

3d Structured Illumination Microscopy, TIRF/Hi-Lo localization microscopy.

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for large-data processing/analysis.

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USER INFORMATION

 If you are interested in trying or using one of our instruments, email us at advmicro(at)vbcf.ac.at to arrange a first meeting/call to discuss your application with our team!

We require acknowledgement of facility use in publications. A simple statement is sufficient and can be placed in the Materials and Methods section or in the Acknowledgments section, depending on the journal format.

Suggested format:
The XXXXXX was performed by the Advanced Microscopy Facility at Vienna BioCenter Core Facilities (VBCF), member of the Vienna BioCenter (VBC), Austria.

In case of (co-)authorship:
The Vienna BioCenter Core Facilities (VBCF) Advanced Microscopy Facility acknowledges funding from the Austrian Federal Ministry of Education, Science & Research; and the City of Vienna.

PUBLICATIONS

Recent progress and current opinions in Brillouin Microscopy for life science application. Antonacci G, Beck T, Bilenca A, Czarske J, Elsayad K, Guck J, Kim K, Krug B, Palombo F, Prevedel R, and Scarcelli G. Biophys Rev. 2020 Jun; 12(3): 615–624.

Mechanical Properties of cellulose fibers measured by Brillouin spectroscopy. Elsayad K, Urstöger G, Czibula C, Teichert C, Gumulec J, Balvan J, Pohlt M, Hirn U. Cellulose 27:4209–4220(2020).

 

Spectral Phasor Analysis for Brillouin Microspectroscopy. Elsayad K.Frontiers in Physics 7:62, 26 April 2019.

β-Catenin–dependent mechanotransduction dates back to the common ancestor of Cnidaria and Bilateria.
Pukhlyakova E, Aman AJ, Elsayad K, Technau U. Proceedings of the National Academy of Sciences115(24), 6231-6236.

Axicon-based Bessel beams for flat-field illumination in total internal reflection fluorescence microscopy.
Schreiber B, Elsayad K, Heinze KG. Optics Letters 42(19):3880-3883 (2017)

A novel non-canonical PIP-box mediates PARG interaction with PCNA
Kaufmann T, Grishkovskaya I, Polyansky AA, Kostrhon S, Kukolj E, Olek KM, Herbert S, Beltzung E, Mechtler K, Peterbauer T, Gotzmann J, Zhang L, Hartl M, Zagrovic B, Elsayad K, Djinovic-Carugo K, Slade D. July 2017 Nucleic Acids Res gkx604

Fluorescence Excitation, Decay, and Energy Transfer in the Vicinity of Thin Dielectric/Metal/Dielectric Layers near Their Surface Plasmon Polariton Cutoff Frequency
Elsayad K and Heinze KG. Chapter 6, Page 111 in "Surface Plasmon Enhanced, Coupled and Controlled Fluorescence" (1st Edition, Edited by C. Geddes).

Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging
Elsayad K, Werner S, Gallemí M, Kong J, Sánchez Guajardo ES, Zhang L, Jaillais Y, Greb T, Belkhadir Y, Sci. Signal. 05 Jul 2016: Vol. 9, Issue 435, pp. rs5

The expression of tubb2b undergoes a developmental transition in murine cortical neurons
Breuss M, Morandell J, Nimpf S, Gstrein T, Lauwers M, Hochstoeger T, Braun A, Chan K, Sánchez Guajardo ES, Zhang L, Suplata M, Heinze KG, Elsayad K, Keays DA, 2015 Journal of Comparative Neurology Volume 523, Issue 15, pages 2161–2186, 15 October 2015

Membrane Protein Localization by SpecON.
Heinze KG, Elsayad K. Imaging & Microscopy16:23

Mal3, the Schizosaccharomyces pombe homolog of EB1, is required for karyogamy and for promoting oscillatory nuclear movement during meiosis
Polakova S, Benko Z, Zhang L, Gregan J Cell Cycle. 13:72

Spectrally coded optical nanosectioning (SpecON) with biocompatible metal–dielectric-coated substrates.
Elsayad K, Urich A, Tan PS, Nemethova M, Small JV, Unterrainer K, Heinze KG. Proc.  Nat. Acad. Sci USA 110:20069

 

ACKNOWLEDGEMENTS

Differential Action of Reelin on Oligomerization of ApoER2 and VLDL Receptor in HEK293 Cells Assessed by Time-Resolved Anisotropy and Fluorescence Lifetime Imaging Microscopy.

Dlugosz P, Tresky R, Nimpf J, Front. Mol. Neurosci., 26 Feb 2019 

p62 filaments capture and present ubiquitinated cargos for autophagy
Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S. The EMBO journal, e98308.

Host-Polarized Cell Growth in Animal Symbionts.
Pende N, Wang J, Weber PM, Verheul J, Kuru E, Rittmann SRK, Leisch N, VanNieuwenhze MS, Brun YV, den Blaauwen T, Bulgheresi S.Current Biology, 28(7), 1039-1051.