Advanced Microscopy

Microscopes that let you see things no one has ever seen before!

 

Optical microscopy has historically played an important role in life-science discovery. Advances in imaging technology are rapidly expanding the boundaries of what is possible, allowing for studies of live cells with unprecedented spatio-temporal resolution and the extraction of diverse biophysical parameters intrinsic to the processes of life. 

The Advanced Microscopy Facility offers a range of cutting-edge, novel and custom optical microscopy techniques, along with associated expertise and assistance, for life science researchers.

Interested in using one of our techniques? Need additional information? Got a specific project question?

SERVICES

Assisted operation of facility instruments & associated data analysis

Following discussion and planning of your project, expert facility staff perform the agreed experiments together with you and assist you with data analysis and interpretation as required for your project. An hourly fee per staff-hour and instrument-hour is charged.

Training on instrument for self-operation (on selected instruments)

For longer-term projects or if you are planning to use an instrument extensively/for several different projects, it may often be desirable to operate the instrument independent of facility staff availability. In this case, training on an instrument by an expert staff member is possibleTraining times will depend on the technique and instrument (estimates provided beforehand). Independent usage on completion of training at trainers’ discretion.

Independent operation of instruments

Instruments may be reserved via our online booking system. We currently have a 24 hr cancellation policy, after which the cost of the respective booked instrument-hours will be charged. Independent operation is charged at an instrument dependent hourly rate.

Consulting (experimental planning/design)

Consulting is provided free of charge if it is tied to the usage or potential usage of an instrument/technique at the facility. General consulting on microscopy related topics is provided at an hourly rate subject to staff availability.

EQUIPMENT

Time-domain FLIM, FLCS, FLCCS, line/imaging FCS, Fluorescence Anisotropy, etc.

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Light sheet microscope

Macro-SPIM, Meso-SPIM, Lattice Light Sheet.

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Confocal Brillouin and Raman scattering.

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3d Structured Illumination Microscopy, TIRF/Hi-Lo localization microscopy.

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for large-data processing/analysis.

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USER INFORMATION

 If you are interested in trying or using one of our instruments, email us at advmicro(at)vbcf.ac.at to arrange a first meeting/call to discuss your application with our team!

To assure funding bodies that we are a worthwhile investment, please remember to acknowledge the facility in all dissemination activities (publications, presentations, etc.) that include data obtained using one of our instruments preferably as follows:

“We acknowledge the Advanced Microscopy Facility of the Vienna BioCenter Core Facilities, member of the Vienna BioCenter Austria, for [service+description of microscope]“.

If a member/members of the facility have contributed scientifically/intellectually to the results or the process of obtaining them, and you would like to acknowledge them as co-authors, please contact us to assure that all relevant funding sources are correctly acknowledged.

PUBLICATIONS

β-Catenin–dependent mechanotransduction dates back to the common ancestor of Cnidaria and Bilateria.
Ekaterina Pukhlyakova, Andrew J. Aman, Kareem Elsayad, and Ulrich Technau. Proceedings of the National Academy of Sciences115(24), 6231-6236.

Axicon-based Bessel beams for flat-field illumination in total internal reflection fluorescence microscopy.
Benjamin Schreiber, Kareem Elsayad, and Katrin G. Heinze. Optics Letters 42(19):3880-3883 (2017)

A novel non-canonical PIP-box mediates PARG interaction with PCNA
Tanja Kaufmann, Irina Grishkovskaya, Anton A. Polyansky, Sebastian Kostrhon, Eva Kukolj, Karin M. Olek, Sebastien Herbert, Etienne Beltzung, Karl Mechtler, Thomas Peterbauer, Josef Gotzmann, Lijuan Zhang, Markus Hartl, Bojan Zagrovic, Kareem Elsayad, Kristina Djinovic-Carugo, Dea Slade. July 2017 Nucleic Acids Res gkx604

Fluorescence Excitation, Decay, and Energy Transfer in the Vicinity of Thin Dielectric/Metal/Dielectric Layers near Their Surface Plasmon Polariton Cutoff Frequency
K. Elsayad and K. Heinze. Chapter 6, Page 111 in "Surface Plasmon Enhanced, Coupled and Controlled Fluorescence" (1st Edition, Edited by C. Geddes).

 

 

 

Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging
Kareem Elsayad, Stephanie Werner, Marçal Gallemí, Jixiang Kong, Edmundo R. Sánchez Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb and Youssef Belkhadir
Sci. Signal. 05 Jul 2016: Vol. 9, Issue 435, pp. rs5

The expression of tubb2b undergoes a developmental transition in murine cortical neurons
Martin Breuss, Jasmin Morandell, Simon Nimpf, Thomas Gstrein, Mattias Lauwers, Tobias Hochstoeger, Andreas Braun, Kelvin Chan, Edmundo R. Sánchez Guajardo, Lijuan Zhang, Marek Suplata, Katrin G. Heinze, Kareem Elsayad and David A. Keays. Journal of Comparative Neurology Volume 523, Issue 15, pages 2161–2186, 15 October 2015

Membrane Protein Localization by SpecON.
K.G.Heinze, K. Elsayad. Imaging & Microscopy16:23

Mal3, the Schizosaccharomyces pombe homolog of EB1, is required for karyogamy and for promoting oscillatory nuclear movement during meiosis
Polakova S., Benko Z., Zhang L., Gregan J. Cell Cycle. 13:72

Spectrally coded optical nanosectioning (SpecON) with biocompatible metal–dielectric-coated substrates.
K. Elsayad, A. Urich, P.S. Tan, M. Nemethova, J.V. Small, K. Unterrainer, K.G.Heinze. Proc.  Nat. Acad. Sci USA 110:20069

 

ACKNOWLEDGEMENTS

p62 filaments capture and present ubiquitinated cargos for autophagy
Gabriele Zaffagnini, Adriana Savova, Alberto Danieli, Julia Romanov, Shirley Tremel, Michael Ebner, Thomas Peterbauer, Martin Sztacho, Riccardo Trapannone, Abul K Tarafder, Carsten Sachse, Sascha Martens. The EMBO journal, e98308.

Host-Polarized Cell Growth in Animal Symbionts.
Pende, Nika, Jinglan Wang, Philipp M. Weber, Jolanda Verheul, Erkin Kuru, K-MR Rittmann Simon, Nikolaus Leisch et al. Current Biology, 28(7), 1039-1051.