Equipment

The microscope is located in Room 4.73.01 (phone: + 43 (1) 7962324 - 7124) and can be reserved via our EM reservation system.

• Hardware:

    Tungsten filament emitter

    100 kV (operated at 80 kV)

    Mega View III CCD camera (Olympus-SIS)

• Software:

    Morgagni software (version 3.0)

    iTEM (version 5.0)

The T20 is located in Room 4.55. and can be reserved via our EM reservation system.

• Hardware:

    LaB6 filament emitter

    Operated at 200 kV

    Eagle 4k HS CCD camera

    Magnetic field compensation system

• Software:

     SerialEM (v3.x): automated data recording; acquisition of tilt series for electron tomography; grid mapping

     Sentinel: monitoring system

The Glacios is located in Room 4.59.02 (phone: + 43 (1) 790 44 - 4267) and can be reserved via our EM reservation system.

• Hardware:

    200 kV X-FEG

    Autoloader for up to 12 cryo samples

    Volta phase-plate

    Ceta Camera

    Falcon3 Direct Electron Detector

    Magnetic field compensation system

• Software:

    Sentinel: monitoring system

    EPU

    SerialEM: automated data recording; acquisition of single particle or tilt series for cryo-electron tomography, and grid mapping

Immersion and slam freezing:

• Leica EM GP: plunge freezer for highly reproducible preparation of cryo-samples. This instrument was developed and tested in a collaboration of the EM Facility (under the lead of the former facility head, Günter Resch) with Leica. A sensor allows for the precise detection of the contact point of liquid sample and filter paper to allow highly-reproducible blotting forces even when filter papers are wrinkled due to a high humidity environment.   
• Manual plunge freezers for vitrification of thin specimens on grids (Room 4.59.02)
• Leica EM CPC metal mirror freezer (Room 4.59.02)

High vacuum evaporators and sputter coaters:

• Edwards Auto306 high vacuum evaporator (Room 4.73.03)
• Bal-Tec MED020 high vacuum evaporator with quartz film thickness monitor for resistance evaporation of metals and carbon, and electron gun evaporation of platinum and carbon (Room 4.73.03)
• BALZERS BAF400 for high vacuum evaporation and freeze etching (Room 4.73.03)
• Bal-Tec SCD005 sputter coater (Room 4.59.02; 4.73.03)

High pressure freezing and freeze substitution:

• Wohlwend HPF Compact 01 high pressure freezer (Room 4.73.04)
• Two Leica AFS A and Leica AFS II automatic freeze substitution devices (Room 4.73.04)

Sectioning:

• Leica EM Trim for trimming resin-embedded specimens (Room 4.73.04)
• Reichert EM Trim
• Reichert UCS ultramicrotome (Room 4.74.02)
• Leica UCT ultramicrotome (Room 4.74.02)
• Leica UCT ultramicrotome with Leica EM FCS cryo-setup (Room 4.74.03)
• Leica RMC, Leica LKB, and Leica EM KMR2 glass knife makers (Room 4.74.02)

Embedding:

• Leica Tissue Processor for embedding larger amounts of tissue (Room 4.74.03)
• Electron Microscopy Sciences 820 microwave (Room 4.74.03)

Sample preparation for scanning electron microscopy:

• BALZERS CPD030 critical point dryer for scanning electron microscopy (Room 4.73.03)
• Bal-Tec SCD050 (Room 4.73.03)

Ancillary equipment:

• Selutec Tec020 cell incubator (Room 4.73.03)
• Two polymerization ovens (Room 4.73.04)
• Double distilled water still (Room 4.73.04)
• Knauer K-7400 semi-micro osmometer (Room 4.73.04)

Light microscopes:

• Two Leica MZ6 stereo microscopes for specimen preparation (Room 4.73.04)
• Leica Wild M3Z stereomicroscope (Room 4.73.03)
• Leica S4E stereomicroscope (Room 4.73.04)