Raster Image Correlation Spectroscopy (RICS)

Instead of correlating the intensity over time, here one also correlates also over space by very rapidly scanning a single detection volume and thus capturing the diffusion of individual molecules. A spatial correlation analysis subsequently allows one to calculate the diffusion coefficient(s).

Examples of use:

• Binding (dynamics) or colocalization of two fluorescently-labelled molecules separated (especially by more >10nm).

Useful reference:

• Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope, Brown CM, et al. (2008), J Microsc. 229(1):78–91.