Fluorescence Anisotropy Imaging Microscopy (FAIM) / Time-Resolved Fluorescence Anisotropy (TRFA) Spectroscopy

Examples of use:

• Binding (dynamics) or colocalization of two fluorescently-labelled molecules separated (especially by more >10nm).

Useful references:

• Fluorescence Lifetime Imaging (FLIM) in Confocal Microscopy Applications: An Overview (Application Note by Picoquant GmbH)
• Fluorescence lifetime imaging microscopy: Spatial resolution of biochemical processes in the cell Bastiaens PIH, Squire A (1999)Trends in Cell Biology, 9:48-52.