Light Sheet Fluorescence Microscopy (LSFM)
Light Sheet Fluorescence Microscopy (LSFM) differs from epi-fluorescence microscopy in that the sample is illuminated at an axis perpendicular to that along which it is detected. In particular the sample is illuminated by an effective so-called “light sheet” which assures that only the region of the sample in the vicinity of the imaged plane is illuminated. There are several advantages to this which are described in more detail here.
At the VBCF we offer 3 types of LSFM setups which cater to different applications that allow for the imaging of entire fixed/cleared brain samples, to the imaging of sub-cellular re-organization dynamics during cell-division at hundreds of frames a second.
- Lattice Light Sheet (LLS) Microscope
- Meso-scale Selective Plain Illumination Microscope, SPIM (L-SPIM)
- Macro-scale Selective Plain Illumination Microscope