How to build an exosome

Inside cells, large multi-subunit machines carry out many essential processes, yet the principles governing their assembly are often poorly understood. Postdoc Tsimafei Navalayeu from the Ameres lab at the Perutz has now mapped the assembly of the RNA exosome in mammalian cells using an inducible CRISPR-based system. The work, published in EMBO Journal, reveals an intricate, stepwise assembly pathway of the multi-subunit exosome, and the quality control mechanisms that regulate its biogenesis.