cryo-EM Symposium

2nd VBC cryo-EM Symposium

The 2nd VBC cryo-EM symposium is a 1-day event that brings together young scientists and experts in the field, to present and share the exciting developments in cryo-EM and its application to biological questions. This year’s symposium will also act as the kick-off day for the first Vienna cryo-EM winter school, which aims to bring cryo-EM to the next generation of scientists (Master, PhD).

cryo-EM Workshop

Cryo-electron microscopy (cryo-EM) is a powerful structural biology technique to study macromolecules in their “native” state. Samples are flash-frozen and imaged under cryogenic conditions in an electron microscope. Computational tools are used to determine three-dimensional densities at near-atomic resolution from noisy data. These densities enable the modeling and refinement of high-quality structural models. Cryo-EM has undergone a revolution in recent years due to developments in both hardware and software. These advances lead to an avalanche of new high-resolution structures and biological insight, ranging from large macromolecular machines such as the proteasome and spliceosome, to small proteins, such as ion channels. Notably, cryo-EM can also reveal the complex dynamics of macromolecules, and thereby yield unique insight into the underlying mechanism. Cryo-EM tomography further enables the structural study of macromolecules directly in cells. Taken together, cryo-EM will likely be a key technique for the next generation of structural biologists.

This course will provide an overview of theoretical concepts and the practical application of cryo-EM single-article analysis and tomography. Experts in cryo-EM will instruct students on theory, lead practical tutorials, and provide examples of state-of-the-art applications of cryo-EM to a variety of biological questions. The course is open to applicants from academia and industry. A distinguishing feature of this course is that it is aimed towards Master and starting PhD students. Applicants must provide a motivation letter to indicate how the course will benefit their research using cryo-EM and that implementation of these plans at their host institute is realistic.

Registration

To participate, please submit your registration via this link.

Agenda Symposium

8.30                    registration 
9.00 - 9.15          opening 
 
9.15  – 10.00      Helen Saibil
10.00 – 10.30     Rafael Leiro Fernandez
10.30 – 11.00     Sebastian Falk
 
11.00 -11.15       coffee break
 
11.15 – 12.00     Nenad Ban
12.00 – 12.30     Franziska Bleichert
12.30 – 12.45     Selected Talk
12.45 - 13.00      Selected Talk     
 
13.00 – 13.45     Lunch break 
13.45 – 14.40     Poster session with coffee
 
14.40 – 15.25     Brenda Schulman 
15.25 – 15.45     Company presentation
15.45 -  16.15     Alexey Amunts
16.15 – 16.45     Clemens Plaschka
 
16.45-17.00        closing
 
Followed by networking/socialising with drinks

Agenda Workshop

15 Feb: Cryo EM Sample preparation
Morning:
Lecture: Sample preparation for cryo EM (David Haselbach)
Sponsor Lecture: Sample preparation (Leica)
Afternoon: 
Sample preparation practical (VBCF Facility) 

16 Feb: Vendor excursion
Evening: workshop dinner

17 Feb: The Electron Microscope
Guest lecture: Electron optics: Chris Russo
Lecture Electron microscope: Thomas Juffmann
Afternoon: 
Practical Electron microscopy: VBCF facility
Evening:
Research lecture: Chris Russo 

18 Feb: Image Processing
Morning: 
Guest lecture: Christos Gatsiogiannis
Webinar Lecture: Sjores Scheres 
Afternoon: 
Practical Image Processing (Clemens Plaschka, David Haselbach, Christos Gatsiogiannis)
Evening: 
Research lecture: Christos Gatsiogiannis

19 Feb: Tomography 
Guest lecture: Electron Tomography Ben Engel
Lecture: Electron Tomography Florian Schur
Afternoon: 
Practical Tomography processing (Florian Schur, VBCF Facility)
Evening 
Research lecture: Ben Engel

20 Feb: Modelling
Morning:
Guest lecture Rosetta for EM Frank DiMaio
Lecture: Modelling: Anton Meinhart
Afternoon: 
Practical: Model Building (Frank Di Maio, Anton Meinhart)
Evening:
Research lecture Frank DiMaio